{"id":19432,"date":"2015-10-07T20:29:10","date_gmt":"2015-10-07T18:29:10","guid":{"rendered":"http:\/\/www.expo21xx.com\/news\/?p=19432"},"modified":"2015-10-07T20:29:10","modified_gmt":"2015-10-07T18:29:10","slug":"fluorescence-lifetime-imaging-pco-film","status":"publish","type":"post","link":"https:\/\/www.expo21xx.com\/news\/fluorescence-lifetime-imaging-pco-film\/","title":{"rendered":"Fluorescence Lifetime Imaging with the pco.film camera"},"content":{"rendered":"<p>PCO introduces the revolutionary new pco.flim camera for biomedical research fluorescence lifetime imaging.\u00a0 Using a new type of image sensor it is suitable for numerous applications in the field of biomedical research.<\/p>\n<div id=\"attachment_19439\" class=\"wp-caption aligncenter\" style=\"width: 690px\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-19439 size-full\" src=\"http:\/\/www.expo21xx.com\/news\/wp-content\/uploads\/pco.flim_.jpg\" alt=\" fluorescence lifetime imaging\" width=\"690\" height=\"546\" srcset=\"https:\/\/www.expo21xx.com\/news\/wp-content\/uploads\/pco.flim_.jpg 690w, https:\/\/www.expo21xx.com\/news\/wp-content\/uploads\/pco.flim_-131x104.jpg 131w, https:\/\/www.expo21xx.com\/news\/wp-content\/uploads\/pco.flim_-350x277.jpg 350w\" sizes=\"auto, (max-width: 690px) 100vw, 690px\" \/><p class=\"wp-caption-text\">pco.flim camera for biomedical research fluorescence lifetime imaging<br \/> <em>Photo by PCO AG<\/em><\/p><\/div>\n<p>The <em>pco.flim<\/em> camera system is the first luminescence lifetime imaging camera using a two tap CMOS image sensor. The camera can internally generate a wide range of frequencies and will also accept external modulation signals within a limited range of frequencies.<\/p>\n<p>It features a variety of trigger input and output signals for easy system integration. Special software provides direct measurements and lifetime analysis.<\/p>\n<p><strong>Technical\u00a0data<\/strong><\/p>\n<p><em>Image sensor<\/em><\/p>\n<ul>\n<li>type of sensor: CMOS<\/li>\n<li>image sensor proprietary<\/li>\n<li>resolution (h x v) 1008 x 1008 pixels<\/li>\n<li>pixel size (h x v) 5.6 \u00b5m x 5.6 \u00b5m<\/li>\n<li>sensor format \/ diagonal 5.7 mm x 5.7 mm \/ 8.1 mm<\/li>\n<li>shutter mode rolling reset \/ global exposure<\/li>\n<li>fullwell capacity 45 000 e- (typ.)<\/li>\n<li>readout noise 45 e- rms (typ.)<\/li>\n<li>dynamic range &gt; 1 000 : 1 (60 dB)<\/li>\n<li>quantum efficiency appr. 39 % @ peak<\/li>\n<li>spectral range visible (tbd)<\/li>\n<li>dark current 1100 e-\/(s.pixel)<\/li>\n<li>DSNU 49 e- rms<\/li>\n<li>PRNU 0.7 %<\/li>\n<\/ul>\n<p><em>Camera<\/em><\/p>\n<ul>\n<li>max. frame rate\u00a0(full frame, full resolution)<\/li>\n<li>90 fps (2 tap readout)<\/li>\n<li>modulation frequency internal 5 kHz &#8211; 40 MHz \/\u00a0external 500 kHz &#8211; 40 MHz<\/li>\n<li>modulation signal shape sinusoidal \/ rectangular<\/li>\n<li>exposure \/ shutter time 10 ns &#8211; 10 s<\/li>\n<li>dynamic range A\/D 14 bit<\/li>\n<li>A\/D conversion factor 3.2 e-\/count<\/li>\n<li>region of interest steps of 16&#215;1 pixel<\/li>\n<li>thermoelectrically cooled + 5 \u00b0C<\/li>\n<li>nonlinearity &lt; 1 %<\/li>\n<li>trigger input signals exposure start\u00a0(phase sequence trigger)<\/li>\n<li>trigger output signals exposure, busy, gate (light enable)<\/li>\n<li>modulation signal output 1 V in 50 W, AC coupled<\/li>\n<li>modulation signal input max. +\/- 5 V in &gt; 1 kW<\/li>\n<li>data interface USB 3.0<\/li>\n<\/ul>\n<p>More detailed information about fluorescence lifetime imaging using the pco.flim camera can be found in the <em>pco.film<\/em> <strong><a href=\"http:\/\/www.pco.de\/fileadmin\/user_upload\/pco-product_sheets\/pco.flim_brochure.pdf\" target=\"_blank\" rel=\"nofollow\">white paper<\/a><\/strong> (as pdf)<\/p>\n<p>The <em>pco.film<\/em> and other cameras can be seen at the<strong>\u00a0<a href=\"http:\/\/www.sfn.org\/annual-meeting\/neuroscience-2015\" target=\"_blank\">Neuroscience 2015<\/a><\/strong>, Chicago, USA taking place 10\/17\/2015 &#8211; 10\/21\/2015. You will find PCO at booth No. 705.<\/p>\n<p>For further details please visit:\u00a0<a href=\"http:\/\/www.expo21xx.com\/link\/redirector.php?url=http:\/\/www.pco.de\" target=\"_blank\">www.pco.de<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PCO introduces the revolutionary new pco.flim camera for biomedical research fluorescence lifetime imaging.\u00a0 Using a new type of image sensor it is suitable for numerous applications in the field of biomedical research. [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":19443,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[352],"tags":[671,194,659,660],"class_list":["post-19432","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-vision-camera","tag-fluorescence-lifetime-imaging","tag-research","tag-scientific-camera","tag-scientific-imaging"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.8.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Fluorescence Lifetime Imaging with the pco.film camera - EXPO21XX.com NEWS<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.expo21xx.com\/news\/fluorescence-lifetime-imaging-pco-film\/\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"MB\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.expo21xx.com\/news\/fluorescence-lifetime-imaging-pco-film\/\",\"url\":\"https:\/\/www.expo21xx.com\/news\/fluorescence-lifetime-imaging-pco-film\/\",\"name\":\"Fluorescence Lifetime Imaging with the pco.film camera - 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