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Fluorescence Lifetime Imaging with the pco.film camera

PCO introduces the revolutionary new pco.flim camera for biomedical research fluorescence lifetime imaging.  Using a new type of image sensor it is suitable for numerous applications in the field of biomedical research.

 fluorescence lifetime imaging

pco.flim camera for biomedical research fluorescence lifetime imaging
Photo by PCO AG

The pco.flim camera system is the first luminescence lifetime imaging camera using a two tap CMOS image sensor. The camera can internally generate a wide range of frequencies and will also accept external modulation signals within a limited range of frequencies.

It features a variety of trigger input and output signals for easy system integration. Special software provides direct measurements and lifetime analysis.

Technical data

Image sensor

  • type of sensor: CMOS
  • image sensor proprietary
  • resolution (h x v) 1008 x 1008 pixels
  • pixel size (h x v) 5.6 µm x 5.6 µm
  • sensor format / diagonal 5.7 mm x 5.7 mm / 8.1 mm
  • shutter mode rolling reset / global exposure
  • fullwell capacity 45 000 e- (typ.)
  • readout noise 45 e- rms (typ.)
  • dynamic range > 1 000 : 1 (60 dB)
  • quantum efficiency appr. 39 % @ peak
  • spectral range visible (tbd)
  • dark current 1100 e-/(s.pixel)
  • DSNU 49 e- rms
  • PRNU 0.7 %

Camera

  • max. frame rate (full frame, full resolution)
  • 90 fps (2 tap readout)
  • modulation frequency internal 5 kHz – 40 MHz / external 500 kHz – 40 MHz
  • modulation signal shape sinusoidal / rectangular
  • exposure / shutter time 10 ns – 10 s
  • dynamic range A/D 14 bit
  • A/D conversion factor 3.2 e-/count
  • region of interest steps of 16×1 pixel
  • thermoelectrically cooled + 5 °C
  • nonlinearity < 1 %
  • trigger input signals exposure start (phase sequence trigger)
  • trigger output signals exposure, busy, gate (light enable)
  • modulation signal output 1 V in 50 W, AC coupled
  • modulation signal input max. +/- 5 V in > 1 kW
  • data interface USB 3.0

More detailed information about fluorescence lifetime imaging using the pco.flim camera can be found in the pco.film white paper (as pdf)

The pco.film and other cameras can be seen at the Neuroscience 2015, Chicago, USA taking place 10/17/2015 – 10/21/2015. You will find PCO at booth No. 705.

For further details please visit: www.pco.de